Primary secondary and tertiary structure of dna pdf

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Primary secondary and tertiary structure of dna pdf

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(As a result, RNA mediates specific recognition and catalytic reactions.) Principles/ideas--RNAs contain characteristic 2° and Nucleic Acid Extinction Coefficient. Replication of DNA. Learning Objectives DNA Structure DNA has three main componentsDeoxyribose (a pentose sugar)Base (there are four different ones)Phosphate DNA structure is often divided into four RNA structure can be specific, stable and complex. The three-dimensional shape of a DNA molecule, or its tertiary structure, is a right-handed double helix (Figure 2b) DNA structure DNA is made up of mol ecul es cal l ed nucleot i des. T he f our t ypes of nitrogen bases are adeni ne (A), t hymine (T), guani ne (G) and cyt osine (C). The most commonly occurring pyrimidines in DNA are cytosine and thymine: Figure Pyramidines This base pairing of nucleotides between the two strands of a single DNA molecule is called DNA’s secondary structure. Rule of Thumb: For double-stranded, plasmid DNA, the extinction coefficient at nm is(μg/mL)cm-1 G must be paired with a C (and vice versa). These A-T and G-C associations are known as complementary base pairs (Fig). Thus, the secondary structure of DNA leads to the hybridization of The secondary structure is composed of the complementary nucleotides bonding and folding together (cytosine to guanine and thymine to adenine) which structure appears as either an alpha or beta pleated sheets. Also sequence isn’t exactly known in many cases. DNA Concentrations: Often measured in μg/mL (or the equivalent ng/μL) instead of M, mM, etc. The tertiary structure is the three-dimensional geometrical formation of the nucleotides and can include B-DNA, A-DNA, and Z-DNA D. Watson and Crick put it all together to solve the structure of DNA in IV. The structure of DNA A. Important features of the DNA structureRight-handed double helixThe helices are antiparallelEach helix has a series of nucleotides held together with phosphodiester bonds between the OH groups in two adjacent sugar residues DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are composed of two different classes of nitrogen-containing bases: the purines and pyrimidines. E ach nucleot i de cont ai ns a phosphate group, a sugar group and a ni t rogen base. The most commonly occurring purines in DNA are adenine and guanine: Figure Purines. The order of these b ases i s what det ermines DNA' s Secondary structure of DNA. Double helix model of DNA. The logic of complementary base pairing.

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