FlyPi

FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).

Difficulté

Medium Moyen

Durée

28 hour(s) heure(s)

Catégories

Science & Biologie

Coût

200 EUR (€)

DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring FlyPi_IMG_7827.jpg en-gb none Creation 0

Introduction

The aim of this tutorial is to consolidate all construction information for this biology lab so I can feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new perspectives, joy, wonder and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators is access to effective scientific tools, likely due to high prices set by development under a patent/scarcity methodology. One way to get around this limiting factor is by building your own biology tools like the FlyPi; An all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, low cost device which allows for better experimental control, customization for specific needs, and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! 

Steps

1- Modularity: show some images/examples of the setup built, Explain Modularity principle .

2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)

3- Building hardware: Explain module flexibilities

4- Software Installation: How to install useful software on SD card

5- Launching FlyPi program: Putting it all together to start collecting image data!

Matériaux

Monitor

Computer mouse - USB

Keyboard - USB

3D print files (.stl) : Under 'Files' tab above

Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )

Outils

Soldering Iron, Solder

Computer with:

- ability to connect SD card

- internet Connection

3D printer

Base Clip.stl

Base.stl

Camera Cap.stl

Camera Housing.stl

CameraServoHolder.stl

LightDiffuser.stl

LightFilter Arm Cross2.stl

LightFilter Arm.STL

ManipulatorBase.stl

Neck L.stl

Neck R.stl

PCBCase Bot.stl

PCBCase Top.stl

RaspberryCase Bot.stl

RaspberryCase Rails.stl

RaspberryCase Top.stl

NeckTriangle Thread.stl

NeckTriangle.stl

RGBHeatSink Arm.stl

RGBHeatSink Frame.stl

RGBHeatSink Hex.stl

RGBRingHolder.stl

Étape 1 - Modularity

1- Modularity: show some images/examples of the setup built, Explain Modularity principle

Étape 2 - Gathering Materials & Creating Parts

2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)

Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):

https://kitspace.org/boards/github.com/prometheus-science/flypi/

3D print files can be found in the files tab

Étape 3 - Building hardware

3- Building hardware: Explain module flexibilities

Étape 4 - Software Installation

4- Software Installation: How to install useful software on SD card

Étape 5 - Launching the FlyPi

5- Launching FlyPi program: Putting it all together to start collecting image data!

Draft