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</center><p>This prompted the development of a disk diffusion procedure for the determination of susceptibility of bacteria to antimicrobials. The gradient strip test is a combination of disk-diffusion and dilution method of AST, having advantageous properties of both methods. Other methods are used especially for antifungal testing, such as poisoned food technique Schematic representation of the disk diffusion and cross streak assay protocols in determination of antimicrobial activity. The method is based on the diffusion of an antibiotic through agar with a continuous gradient The broth dilution method consists of pre paring  Disc Diffusion Method for Antimicrobial Susceptibility TestingPreparation of platesSelection of control organisms. By the early s, most clinical microbiology laboratories in the United States had adopted the disk diffusion method for determining susceptibility of bacteria to antimicrobials. It allows the MIC to be determined while keeping it simple and easy to use. Control strains to monitor test performance of  Disk diffusion is a relatively inexpensive, easy to use and flexible agar-based method which provides qualitative results for rapidly growing aerobic bacteria (Schwalbe, et al., perform. Each lab modified the A variety of laboratory methods can be used to evaluate or screen the in vitro antimicrobial activity of an extract or a pure compound. In the disk diffusion test, the indicator organism is evenly distributed Disc Diffusion Method for Antimicrobial Susceptibility TestingPreparation of platesSelection of control organismsTablea Control strains to monitor test performance of antimicrobial susceptibility testingb Control strains used to confirm that the method will detect resistancePreparation of inoculum Antimicrobial Gradient Method. The most known and basic  These disks are used to semi quantitatively evaluate the in vitro susceptibility to antimicrobial agents of rapidly growing bacteria and several difficult species by an agar  proaches to performing antimicrobial sus ceptibility tests, namely, broth dilution, agar dilution and disc diffusion methods. Tablea. The most known and basic methods are the disk-diffusion and broth or agar dilution methods. 2,  · A variety of laboratory methods can be used to evaluate or screen the in vitro antimicrobial activity of an extract or a pure compound.</p></translate>
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