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Biophysical Equipment GroupCommunity Communauté

https://www.youtube.com/@Neurobiophysics/videos ; https://vimeo.com/user209330965

Leninsky pr. 38-2, room 18, INEPCP RAS, Moscow neurobiophys@gmail.com https://www.youtube.com/@Neurobiophysics/videos

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CCD-or CMOS-lab-on-a-chip based on discrete converters of different physical and chemical parameters of samples into the optical signals with positional sensitivity for morphometry of non-optical patterns

A novel ultra-compact analytical system for simultaneous analysis of a variety of physical and chemical parameters of different biological samples (medical or veterinary analytes, food, soil microbiota, hydrobiological water probes etc.) both in the field and laboratory conditions has been developed on the basis of a single multiparametric microchip with a set of replaceable cartridges / converters, which allows to perform a complex diagnostic procedure of the analyte. The prototypes of automatic microfluidic devices for multiparametric mapping of biochemical and synthetic analytes using planar converters-visualizers have been designed. We propose to use a lab-on-a-chip with a number of replaceable cartridges-converters of various physical parameters / variables to optical signals, which can be detected using CMOS or CCD, as a single analytic and microelectronic platform for mapping and visualization of different physical and chemical parameters. The above system allows to perform a simultaneous mapping, detection and visualization of a number of the sample characteristics, such as: magnetic field (using magnetic film indicators and flux-detectors); electrochemical parameters (potentiometric indicators); laser beam transmission outside the visible spectral range (using the doped solid matrices); distribution of the emitting regions in autoradiography (using the scintillation detectors with different quenching factors or "position-sensitive spinthariscopes"); polarization characteristics and the angular fluorescence polarization (using polaroid films with the rotation angles changed by the stepper motors); the local biothermogenesis temperature of the sample at different points on a chip (according to the NIR-HDRI thermography principles), etc.

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Oleg Gradov

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DIALACTRON TH2 (Configuration: DROPLETRON)

Device for the morphometric and photometric measurements of the sample droplets supplied from the pipette / micropipette tips and cuvettes/ cells (2017-2020). This setup can be used with the permanent monitoring of temperature and humidity in the cell and in the external medium [1].

References: [1].

Orekhov, T. K. and Gradov, O. V. (2021). Digital spectrozonal and multispectral lens-less devices with spectrophotometric temperature calibration guis for dairy farming and qualimetry of diary products. Lecture Notes in Networks and Systems, volume 228, pages 300–324. DOI: 10.1007/978-3-030-77448-6_29

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DIALACTRON _ POLAR (Configuration P1)

Instruments for polarizing lens-less microscopy and microphotometry of anisotropic diary or other crystallized media in dehydration / rehydration tests (“facial structures”) [1].

References:

• [1]. Orekhov, T. K. and Gradov, O. V. (2021). Digital spectrozonal and multispectral lens-less devices with spectrophotometric temperature calibration guis for dairy farming and qualimetry of diary products. Lecture Notes in Networks and Systems, volume 228, pages 300–324. DOI: 10.1007/978-3-030-77448-6_29

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Lens-Less Microscopes with 206 nm and 254 nm interference filters

Lens-Less Microscopes with 206 nm and 254 nm filters (design: O. Gradov, F. Orehov) [2].

References:

• [2]. Orekhov, F. K. and Gradov, O. V. (2023). Towards ultraviolet microbeam scanning and lens-less UV microbeam microscopy with mirror galvanometric scanners: From the history of research instrumentation to engineering of modern mechatronic optical systems. Journal of Sensor Networks and Data Communications, 3(1):117–137 (Invited Paper).

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DIALACTRON _ POLAR (Configuration P94)

Instruments for polarizing lens-less microscopy and microphotometry (see above mentioned review paper) :

• [1]. Orekhov, T. K. and Gradov, O. V. (2021). Digital spectrozonal and multispectral lens-less devices with spectrophotometric temperature calibration guis for dairy farming and qualimetry of diary products. Lecture Notes in Networks and Systems, volume 228, pages 300–324. DOI: 10.1007/978-3-030-77448-6_29).

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Multiparametric Lens-Less Granulometer and Filter Tester

Basic idea proposed in the paper:

• [3] Gradov, O. V. and Jablokov, A. G. (2016). Novel morphometrics-on-a-chip: CCD- or CMOS-lab-on-a-chip based on discrete converters of different physical and chemical parameters of histological samples into the optical signals with positional sensitivity for morphometry of non-optical patterns. Journal of Biomedical Technologies, (2):1–29.

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DIALACTRON (Configuration: CY-F3)

DIALACTRON (Configuration: CY-F3). Lens-less istrument for cylinder plastic photometric cells with the flexible extensible cell fixer for transfocator-like functioning in different regimes of photometric illumination for external light source positioning.

References:

• [1]. Orekhov, T. K. and Gradov, O. V. (2021). Digital spectrozonal and multispectral lens-less devices with spectrophotometric temperature calibration guis for dairy farming and qualimetry of diary products. Lecture Notes in Networks and Systems, volume 228, pages 300–324. DOI: 10.1007/978-3-030-77448-6_29

If you wish like to visit us, please contact us via e-mail: neurobiophys[at]gmail.com or o.v.gradov[at]gmail.com.

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DIALACTRON-NEPHELOTRON

DIALACTRON-NEPHELOTRON : Laser lens-less microscope-photometer and nephelometer with the compact UV-A diode laser source (2016-2018).

• [1]. Orekhov, T. K. and Gradov, O. V. (2021). Digital spectrozonal and multispectral lens-less devices with spectrophotometric temperature calibration guis for dairy farming and qualimetry of diary products. Lecture Notes in Networks and Systems, volume 228, pages 300–324. DOI: 10.1007/978-3-030-77448-6_29

If you wish like to visit us, please contact us via e-mail: neurobiophys[at]gmail.com or o.v.gradov[at]gmail.com.

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Setup for nanosecond testing of CCD-based optofluidic lens-less microscopes

See video:

If you wish like to visit us, please contact us via e-mail: neurobiophys[at]gmail.com or o.v.gradov[at]gmail.com.

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Multiparametric reader system for multidescriptor mapping of biological samples

The first version of the multiparametric reader system for multidescriptor mapping of biological samples.

The second version of the multiparametric reader system for multidescriptor mapping of biological samples.

Redox-signal conversion into the optical signal using colorimetric / spectrocolorimetric “chemical pixels” (“chemical resells” / “sensels”): A,C – baseline (reactive film without detective dye response); B,D – indicator signal. Equivalent mosaic sensor-converter elements may be assembled using different converters and indicators (not only “pH-pixels” / “pХ-pixels”, but also “electric luminescence pixels”, “magnetic field pixels”, “radiation pixels”).

• [4] Gradov, O. V. and Jablokov, A. G. (2016). Novel morphometrics-on-a-chip: CCD- or CMOS-lab-on-a-chip based on discrete converters of different physical and chemical parameters of histological samples into the optical signals with positional sensitivity for morphometry of non-optical patterns. Journal of Biomedical Technologies, (2):1–29.

If you wish like to visit us, please contact us via e-mail: neurobiophys[at]gmail.com or o.v.gradov[at]gmail.com.

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Ultracompact lab-on-a-chip reader with USB and TRS.

Ultracompact lab-on-a-chip reader with USB and TRS.

• [5] Gradov, O. V. and Jablokov, A. G. (2016). Novel morphometrics-on-a-chip: CCD- or CMOS-lab-on-a-chip based on discrete converters of different physical and chemical parameters of histological samples into the optical signals with positional sensitivity for morphometry of non-optical patterns. Journal of Biomedical Technologies, (2):1–29.

If you wish like to visit us, please contact us via e-mail: neurobiophys[at]gmail.com or o.v.gradov[at]gmail.com.

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CCD-or CMOS-lab-on-a-chip based on discrete converters of different physical and chemical parameters of samples into the optical signals with positional sensitivity for morphometry of non-optical patterns

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