| Ligne 2 : | Ligne 2 : | ||
|Main_Picture=FlyPi_IMG_7640.JPG | |Main_Picture=FlyPi_IMG_7640.JPG | ||
|Licences=Attribution (CC BY) | |Licences=Attribution (CC BY) | ||
| − | |Description=FlyPi is a 'Do-It-Yourself' all in one biology lab tool that offers incredible versitility with optical microscopy, diagnostics, state of the art methods of research in neurosciences like optogenetics, calcium Imaging and fluorescence microscopy (Maia Chagas, 2017) among many other possibilities. | + | |Description=<translate>FlyPi is a 'Do-It-Yourself' all in one biology lab tool that offers incredible versitility with optical microscopy, diagnostics, state of the art methods of research in neurosciences like optogenetics, calcium Imaging and fluorescence microscopy (Maia Chagas, 2017) among many other possibilities.</translate> |
|Area=Science and Biology | |Area=Science and Biology | ||
|Type=Creation | |Type=Creation | ||
| Ligne 16 : | Ligne 16 : | ||
}} | }} | ||
{{ {{tntn|Introduction}} | {{ {{tntn|Introduction}} | ||
| − | |Introduction=In this tutorial, I share the journey of how I built the FlyPi, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! | + | |Introduction=<translate>In this tutorial, I share the journey of how I built the FlyPi, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! |
Steps | Steps | ||
| Ligne 30 : | Ligne 30 : | ||
5- Software Installation: How to install interface and useful imagej FIJI software on SD card | 5- Software Installation: How to install interface and useful imagej FIJI software on SD card | ||
| − | 6- Launching the FlyPi: Putting it all together to start collecting image data! | + | 6- Launching the FlyPi: Putting it all together to start collecting image data!</translate> |
}} | }} | ||
{{ {{tntn|Materials}} | {{ {{tntn|Materials}} | ||
|Step_Picture_00=FlyPi_IMG_7643.JPG | |Step_Picture_00=FlyPi_IMG_7643.JPG | ||
| − | |Material=Monitor | + | |Material=<translate>Monitor |
Computer mouse - USB | Computer mouse - USB | ||
| Ligne 42 : | Ligne 42 : | ||
3D print files (.stl) : Under 'Files' tab above | 3D print files (.stl) : Under 'Files' tab above | ||
| − | Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ ) | + | Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )</translate> |
| − | |Tools=Soldering Iron, Solder | + | |Tools=<translate>Soldering Iron, Solder |
Computer with: | Computer with: | ||
| Ligne 51 : | Ligne 51 : | ||
- internet Connection | - internet Connection | ||
| − | 3D printer | + | 3D printer</translate> |
|Tuto_Attachments={{ {{tntn|Tuto Attachments}} | |Tuto_Attachments={{ {{tntn|Tuto Attachments}} | ||
|Attachment=Base Clip.stl | |Attachment=Base Clip.stl | ||
| Ligne 100 : | Ligne 100 : | ||
{{ {{tntn|Separator}}}} | {{ {{tntn|Separator}}}} | ||
{{ {{tntn|Tuto Step}} | {{ {{tntn|Tuto Step}} | ||
| − | |Step_Title=Understanding Modularity | + | |Step_Title=<translate>Understanding Modularity</translate> |
| − | |Step_Content=1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. | + | |Step_Content=<translate>1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. |
The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. The modules in the PCB are Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. | The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. The modules in the PCB are Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. | ||
| − | To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial). | + | To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).</translate> |
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG | |Step_Picture_00=FlyPi_IMG_7835_1_.JPG | ||
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| ||
| Ligne 114 : | Ligne 114 : | ||
}} | }} | ||
{{ {{tntn|Tuto Step}} | {{ {{tntn|Tuto Step}} | ||
| − | |Step_Title=Gathering Materials & Creating Parts | + | |Step_Title=<translate>Gathering Materials & Creating Parts</translate> |
| − | |Step_Content=2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. | + | |Step_Content=<translate>2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. |
* Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts): | * Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts): | ||
https://kitspace.org/boards/github.com/prometheus-science/flypi/ | https://kitspace.org/boards/github.com/prometheus-science/flypi/ | ||
| − | * 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived. | + | * 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.</translate> |
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png | |Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png | ||
}} | }} | ||
{{ {{tntn|Tuto Step}} | {{ {{tntn|Tuto Step}} | ||
| − | |Step_Title=Building PCB board | + | |Step_Title=<translate>Building PCB board</translate> |
| − | |Step_Content= | + | |Step_Content=<translate>The PCB board is organized in the following modules: |
| − | |Step_Picture_00= | + | |
| + | a) power 1 - 12v to 5v converter dedicated to power the Raspberry Pi | ||
| + | |||
| + | b) power 2 - 12v to 5v converter to power LEDs, LED ring, Thermistor and devices on the I2C port | ||
| + | |||
| + | c) High power LED / 5V general module 1 - Port that can be used with high power LEDs or any other component that takes up to 5V (uses a NPN transistor, PWM capable). | ||
| + | |||
| + | d) High power LED / 5V general module 2 - Port that can be used with high power LEDs or any other component that takes up to 5V. | ||
| + | |||
| + | e) LED ring module - used to power and control the LED ring | ||
| + | |||
| + | f) servo motor module - used to power and control a continuous servo motor | ||
| + | |||
| + | g) Peltier element module - comprised of fan, RGB led, thermistor and peltier element ports. | ||
| + | |||
| + | A fully populated port should look like the one in the image</translate> | ||
| + | |Step_Picture_00=FlyPi_all_modules.jpg | ||
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| + | |Step_Picture_01=FlyPi_IMG_20181106_162732387.jpg | ||
| + | |Step_Picture_02=FlyPi_IMG_20181106_165211238.jpg | ||
}} | }} | ||
{{ {{tntn|Tuto Step}} | {{ {{tntn|Tuto Step}} | ||
| − | |Step_Title=Adding Modules | + | |Step_Title=<translate>Adding Modules</translate> |
| − | |Step_Content=4- Software Installation: How to install useful software on SD card | + | |Step_Content=<translate>4- Software Installation: How to install useful software on SD card</translate> |
}} | }} | ||
{{ {{tntn|Tuto Step}} | {{ {{tntn|Tuto Step}} | ||
| − | |Step_Title=Launching the FlyPi | + | |Step_Title=<translate>Launching the FlyPi</translate> |
| − | |Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data! | + | |Step_Content=<translate>5- Launching FlyPi program: Putting it all together to start collecting image data!</translate> |
}} | }} | ||
{{ {{tntn|Notes}} | {{ {{tntn|Notes}} | ||
| − | |Notes= | + | |Notes=<translate></translate> |
}} | }} | ||
{{ {{tntn|Tuto Status}} | {{ {{tntn|Tuto Status}} | ||
| − | |Complete= | + | |Complete=Published |
}} | }} | ||
Version du 11 novembre 2018 à 00:49
FlyPi is a 'Do-It-Yourself' all in one biology lab tool that offers incredible versitility with optical microscopy, diagnostics, state of the art methods of research in neurosciences like optogenetics, calcium Imaging and fluorescence microscopy (Maia Chagas, 2017) among many other possibilities.
Difficulté
Moyen
Durée
28 heure(s)
Catégories
Science & Biologie
Coût
100 EUR (€)
Introduction
In this tutorial, I share the journey of how I built the FlyPi, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems!
Steps
1- Understanding Modularity: How to decide which build is needed
2- Gathering materials & Creating parts: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)
3- Building PCB hardware: Quick overview of the PCB build. Note the space left due to the absence of the servo motor for the (x,y) axis movement.
4- Combining 3D printed body: Modular flexibility in use.
5- Software Installation: How to install interface and useful imagej FIJI software on SD card
6- Launching the FlyPi: Putting it all together to start collecting image data!
Erreur lors de la création de la miniature : convert: unable to extend cache `/var/www/dokitfarm/sites/Wikifab/images/b/b6/FlyPi_IMG_7643.JPG': File too large @ error/cache.c/OpenPixelCache/4006. convert: no images defined `/tmp/transform_496c3532bc08.jpg' @ error/convert.c/ConvertImageCommand/3258. Error code: 1
Matériaux
Monitor
Computer mouse - USB
Keyboard - USB
3D print files (.stl) : Under 'Files' tab above
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )
Outils
Soldering Iron, Solder
Computer with:
- ability to connect SD card
- internet Connection
3D printer
Étape 1 - Understanding Modularity
1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose.
The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. The modules in the PCB are Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point.
To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).
Étape 2 - Gathering Materials & Creating Parts
2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs.
- Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):
https://kitspace.org/boards/github.com/prometheus-science/flypi/
- 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.
Étape 3 - Building PCB board
The PCB board is organized in the following modules:
a) power 1 - 12v to 5v converter dedicated to power the Raspberry Pi
b) power 2 - 12v to 5v converter to power LEDs, LED ring, Thermistor and devices on the I2C port
c) High power LED / 5V general module 1 - Port that can be used with high power LEDs or any other component that takes up to 5V (uses a NPN transistor, PWM capable).
d) High power LED / 5V general module 2 - Port that can be used with high power LEDs or any other component that takes up to 5V.
e) LED ring module - used to power and control the LED ring
f) servo motor module - used to power and control a continuous servo motor
g) Peltier element module - comprised of fan, RGB led, thermistor and peltier element ports.
A fully populated port should look like the one in the image
Étape 4 - Adding Modules
4- Software Installation: How to install useful software on SD card
Étape 5 - Launching the FlyPi
5- Launching FlyPi program: Putting it all together to start collecting image data!
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