https://wikifab.org/w/api.php?action=feedcontributions&feedformat=atom&user=TinkererTalleyWikifab - Contributions de l’utilisateur [fr]2026-06-19T20:28:25ZContributions de l’utilisateurMediaWiki 1.31.3https://wikifab.org/w/index.php?title=FlyPi&diff=80528FlyPi2020-03-13T15:33:33Z<p>TinkererTalley : </p>
<hr />
<div>{{Tuto Details<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=<translate><!--T:1--><br />
FlyPi is a 'Do-It-Yourself' all in one biology lab tool that offers incredible versitility with optical microscopy, diagnostics, state of the art methods of research in neurosciences like optogenetics, calcium Imaging and fluorescence microscopy (Maia Chagas, 2017) among many other possibilities.</translate><br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{Introduction<br />
|Introduction=<translate><!--T:2--><br />
In this tutorial, I share the journey of how I built the FlyPi, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life usually unobserved. This is an amazing experience for anyone curious of what lies beneath our immediate visual capabilities. One main constraint scientists at all levels (Students, researchers and/or educators) is access to effective scientific tools. The cost of tools as well as repait and maintenance, not to mention calibration. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
<!--T:40--><br />
Steps<br />
<br />
<!--T:41--><br />
1- Understanding Modularity: Deciding what to include or exclude in the build<br />
<br />
<!--T:42--><br />
2- Gathering materials & Creating parts: Options to buy parts from kitspace, link to original github repository<br />
<br />
<!--T:43--><br />
Due to modularity, not all parts are necessary for basic functionality. (Repository has more detailed bill of material files)<br />
<br />
<!--T:44--><br />
3- Building PCB hardware: Quick overview of the PCB build<br />
<br />
<!--T:45--><br />
4- Software Installation: How to install interface and useful imagej FIJI software on SD card<br />
<br />
<!--T:46--><br />
5- Launching the FlyPi: Putting it all together to start collecting image data!</translate><br />
}}<br />
{{Materials<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=<translate><!--T:10--><br />
Monitor<br />
<br />
<!--T:11--><br />
Computer mouse - USB<br />
<br />
<!--T:12--><br />
Keyboard - USB<br />
<br />
<!--T:13--><br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
<!--T:14--><br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )</translate><br />
|Tools=<translate><!--T:15--><br />
Soldering Iron, Solder<br />
<br />
<!--T:16--><br />
Computer with:<br />
<br />
<!--T:17--><br />
- SD card reader<br />
<br />
<!--T:18--><br />
- Internet connection<br />
<br />
<!--T:19--><br />
3D printer</translate><br />
|Tuto_Attachments={{Tuto Attachments<br />
|Attachment=Base Clip.stl<br />
}}{{Tuto Attachments<br />
|Attachment=Base.stl<br />
}}{{Tuto Attachments<br />
|Attachment=Camera Cap.stl<br />
}}{{Tuto Attachments<br />
|Attachment=Camera Housing.stl<br />
}}{{Tuto Attachments<br />
|Attachment=CameraServoHolder.stl<br />
}}{{Tuto Attachments<br />
|Attachment=LightDiffuser.stl<br />
}}{{Tuto Attachments<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{Tuto Attachments<br />
|Attachment=LightFilter Arm.STL<br />
}}{{Tuto Attachments<br />
|Attachment=ManipulatorBase.stl<br />
}}{{Tuto Attachments<br />
|Attachment=Neck L.stl<br />
}}{{Tuto Attachments<br />
|Attachment=Neck R.stl<br />
}}{{Tuto Attachments<br />
|Attachment=PCBCase Bot.stl<br />
}}{{Tuto Attachments<br />
|Attachment=PCBCase Top.stl<br />
}}{{Tuto Attachments<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{Tuto Attachments<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{Tuto Attachments<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{Tuto Attachments<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{Tuto Attachments<br />
|Attachment=NeckTriangle.stl<br />
}}{{Tuto Attachments<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{Tuto Attachments<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{Tuto Attachments<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{Tuto Attachments<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{Tuto Step<br />
|Step_Title=<translate><!--T:20--><br />
Understanding Modularity</translate><br />
|Step_Content=<translate><!--T:21--><br />
1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose.<br />
<br />
<!--T:22--><br />
The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. Modules include the Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point.<br />
<br />
<!--T:23--><br />
To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).</translate><br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{Tuto Step<br />
|Step_Title=<translate><!--T:24--><br />
Gathering Materials & Creating Parts</translate><br />
|Step_Content=<translate><!--T:25--><br />
2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. <br />
* Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
* 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.</translate><br />
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png<br />
}}<br />
{{Tuto Step<br />
|Step_Title=<translate><!--T:26--><br />
Building PCB board</translate><br />
|Step_Content=<translate><!--T:27--><br />
The PCB board is organized in the following modules:<br />
<br />
<!--T:28--><br />
a) power 1 - 12v to 5v converter dedicated to power the Raspberry Pi<br />
<br />
<!--T:29--><br />
b) power 2 - 12v to 5v converter to power LEDs, LED ring, Thermistor and devices on the I2C port<br />
<br />
<!--T:30--><br />
c) High power LED / 5V general module 1 - Port that can be used with high power LEDs or any other component that takes up to 5V (uses a NPN transistor, PWM capable).<br />
<br />
<!--T:31--><br />
d) High power LED / 5V general module 2 - Port that can be used with high power LEDs or any other component that takes up to 5V.<br />
<br />
<!--T:32--><br />
e) LED ring module - used to power and control the LED ring<br />
<br />
<!--T:33--><br />
f) servo motor module - used to power and control a continuous servo motor<br />
<br />
<!--T:34--><br />
g) Peltier element module - comprised of fan, RGB led, thermistor and peltier element ports.<br />
<br />
<!--T:35--><br />
A fully populated port should look like the one in the image</translate><br />
|Step_Picture_00=FlyPi_all_modules.jpg<br />
|Step_Picture_00_annotation={"objects":[{"type":"textbox","originX":"center","originY":"center","left":353.99,"top":252,"width":95.97,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"A","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } 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},{"type":"textbox","originX":"center","originY":"center","left":49,"top":183,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"E","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } },{"type":"textbox","originX":"center","originY":"center","left":58,"top":224,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"F","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} },"1":{"0":{} } } },{"type":"textbox","originX":"center","originY":"center","left":59,"top":334,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"G","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } }],"height":"571","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_20181106_162732387.jpg<br />
|Step_Picture_02=FlyPi_IMG_20181106_165211238.jpg<br />
}}<br />
{{Tuto Step<br />
|Step_Title=<translate><!--T:36--><br />
Adding Modules</translate><br />
|Step_Content=<translate><!--T:37--><br />
4- Software Installation: How to install useful software on SD card</translate><br />
}}<br />
{{Tuto Step<br />
|Step_Title=<translate><!--T:38--><br />
Launching the FlyPi</translate><br />
|Step_Content=<translate><!--T:39--><br />
5- Launching FlyPi program: Putting it all together to start collecting image data!</translate><br />
}}<br />
{{Notes<br />
|Notes=<translate></translate><br />
}}<br />
{{PageLang<br />
}}<br />
{{Tuto Status<br />
|Complete=Published<br />
}}<br />
{{Separator}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=56917FlyPi2018-11-22T14:55:00Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=<translate><!--T:1--> FlyPi is a 'Do-It-Yourself' all in one biology lab tool that offers incredible versitility with optical microscopy, diagnostics, state of the art methods of research in neurosciences like optogenetics, calcium Imaging and fluorescence microscopy (Maia Chagas, 2017) among many other possibilities.</translate><br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=<translate><!--T:2--><br />
In this tutorial, I share the journey of how I built the FlyPi, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
<!--T:40--><br />
Steps<br />
<br />
<!--T:41--><br />
1- Understanding Modularity: Deciding what to include or exclude in the build<br />
<br />
<!--T:42--><br />
2- Gathering materials & Creating parts: Options to buy parts from kitspace, link to original github repository<br />
<br />
<!--T:43--><br />
Due to modularity, not all parts are necessary for basic functionality. (Repository has more detailed bill of material files)<br />
<br />
<!--T:44--><br />
3- Building PCB hardware: Quick overview of the PCB build<br />
<br />
<!--T:45--><br />
4- Software Installation: How to install interface and useful imagej FIJI software on SD card<br />
<br />
<!--T:46--><br />
5- Launching the FlyPi: Putting it all together to start collecting image data!</translate><br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=<translate><!--T:10--><br />
Monitor <br />
<br />
<!--T:11--><br />
Computer mouse - USB<br />
<br />
<!--T:12--><br />
Keyboard - USB<br />
<br />
<!--T:13--><br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
<!--T:14--><br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )</translate><br />
|Tools=<translate><!--T:15--><br />
Soldering Iron, Solder<br />
<br />
<!--T:16--><br />
Computer with:<br />
<br />
<!--T:17--><br />
- SD card reader <br />
<br />
<!--T:18--><br />
- Internet connection<br />
<br />
<!--T:19--><br />
3D printer</translate><br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
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|Attachment=NeckTriangle Thread.stl<br />
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|Attachment=NeckTriangle.stl<br />
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|Attachment=RGBHeatSink Frame.stl<br />
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|Attachment=RGBRingHolder.stl<br />
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}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:20--> Understanding Modularity</translate><br />
|Step_Content=<translate><!--T:21--><br />
1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
<!--T:22--><br />
The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. Modules include the Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
<!--T:23--><br />
To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).</translate><br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:24--> Gathering Materials & Creating Parts</translate><br />
|Step_Content=<translate><!--T:25--><br />
2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. <br />
* Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
* 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.</translate><br />
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:26--> Building PCB board</translate><br />
|Step_Content=<translate><!--T:27--><br />
The PCB board is organized in the following modules:<br />
<br />
<!--T:28--><br />
a) power 1 - 12v to 5v converter dedicated to power the Raspberry Pi<br />
<br />
<!--T:29--><br />
b) power 2 - 12v to 5v converter to power LEDs, LED ring, Thermistor and devices on the I2C port<br />
<br />
<!--T:30--><br />
c) High power LED / 5V general module 1 - Port that can be used with high power LEDs or any other component that takes up to 5V (uses a NPN transistor, PWM capable).<br />
<br />
<!--T:31--><br />
d) High power LED / 5V general module 2 - Port that can be used with high power LEDs or any other component that takes up to 5V.<br />
<br />
<!--T:32--><br />
e) LED ring module - used to power and control the LED ring<br />
<br />
<!--T:33--><br />
f) servo motor module - used to power and control a continuous servo motor<br />
<br />
<!--T:34--><br />
g) Peltier element module - comprised of fan, RGB led, thermistor and peltier element ports.<br />
<br />
<!--T:35--><br />
A fully populated port should look like the one in the image</translate><br />
|Step_Picture_00=FlyPi_all_modules.jpg<br />
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},{"type":"textbox","originX":"center","originY":"center","left":58,"top":224,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"F","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} },"1":{"0":{} } } 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|Step_Picture_01=FlyPi_IMG_20181106_162732387.jpg<br />
|Step_Picture_02=FlyPi_IMG_20181106_165211238.jpg<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:36--> Adding Modules</translate><br />
|Step_Content=<translate><!--T:37--> 4- Software Installation: How to install useful software on SD card</translate><br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:38--> Launching the FlyPi</translate><br />
|Step_Content=<translate><!--T:39--> 5- Launching FlyPi program: Putting it all together to start collecting image data!</translate><br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<translate></translate><br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Published<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=56905FlyPi2018-11-22T14:53:40Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=<translate><!--T:1--> FlyPi is a 'Do-It-Yourself' all in one biology lab tool that offers incredible versitility with optical microscopy, diagnostics, state of the art methods of research in neurosciences like optogenetics, calcium Imaging and fluorescence microscopy (Maia Chagas, 2017) among many other possibilities.</translate><br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=<translate><!--T:2--><br />
In this tutorial, I share the journey of how I built the FlyPi, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Understanding Modularity: How to decide which build is needed<br />
<br />
2- Gathering materials & Creating parts: Options to buy parts from kitspace, link to original github repository<br />
<br />
Due to modularity, not all parts are necessary for basic functionality. (Repository has more detailed bill of material files)<br />
<br />
3- Building PCB hardware: Quick overview of the PCB build<br />
<br />
4- Software Installation: How to install interface and useful imagej FIJI software on SD card<br />
<br />
5- Launching the FlyPi: Putting it all together to start collecting image data!</translate><br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=<translate><!--T:10--><br />
Monitor <br />
<br />
<!--T:11--><br />
Computer mouse - USB<br />
<br />
<!--T:12--><br />
Keyboard - USB<br />
<br />
<!--T:13--><br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
<!--T:14--><br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )</translate><br />
|Tools=<translate><!--T:15--><br />
Soldering Iron, Solder<br />
<br />
<!--T:16--><br />
Computer with:<br />
<br />
<!--T:17--><br />
- SD card reader <br />
<br />
<!--T:18--><br />
- Internet connection<br />
<br />
<!--T:19--><br />
3D printer</translate><br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
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|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
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|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
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|Attachment=PCBCase Top.stl<br />
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|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:20--> Understanding Modularity</translate><br />
|Step_Content=<translate><!--T:21--><br />
1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
<!--T:22--><br />
The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. Modules include the Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
<!--T:23--><br />
To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).</translate><br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:24--> Gathering Materials & Creating Parts</translate><br />
|Step_Content=<translate><!--T:25--><br />
2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. <br />
* Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
* 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.</translate><br />
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:26--> Building PCB board</translate><br />
|Step_Content=<translate><!--T:27--><br />
The PCB board is organized in the following modules:<br />
<br />
<!--T:28--><br />
a) power 1 - 12v to 5v converter dedicated to power the Raspberry Pi<br />
<br />
<!--T:29--><br />
b) power 2 - 12v to 5v converter to power LEDs, LED ring, Thermistor and devices on the I2C port<br />
<br />
<!--T:30--><br />
c) High power LED / 5V general module 1 - Port that can be used with high power LEDs or any other component that takes up to 5V (uses a NPN transistor, PWM capable).<br />
<br />
<!--T:31--><br />
d) High power LED / 5V general module 2 - Port that can be used with high power LEDs or any other component that takes up to 5V.<br />
<br />
<!--T:32--><br />
e) LED ring module - used to power and control the LED ring<br />
<br />
<!--T:33--><br />
f) servo motor module - used to power and control a continuous servo motor<br />
<br />
<!--T:34--><br />
g) Peltier element module - comprised of fan, RGB led, thermistor and peltier element ports.<br />
<br />
<!--T:35--><br />
A fully populated port should look like the one in the image</translate><br />
|Step_Picture_00=FlyPi_all_modules.jpg<br />
|Step_Picture_00_annotation={"objects":[{"type":"textbox","originX":"center","originY":"center","left":353.99,"top":252,"width":95.97,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"A","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } 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},{"type":"textbox","originX":"center","originY":"center","left":49,"top":183,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"E","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } },{"type":"textbox","originX":"center","originY":"center","left":58,"top":224,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"F","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} },"1":{"0":{} } } },{"type":"textbox","originX":"center","originY":"center","left":59,"top":334,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"G","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } }],"height":"571","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_20181106_162732387.jpg<br />
|Step_Picture_02=FlyPi_IMG_20181106_165211238.jpg<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:36--> Adding Modules</translate><br />
|Step_Content=<translate><!--T:37--> 4- Software Installation: How to install useful software on SD card</translate><br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:38--> Launching the FlyPi</translate><br />
|Step_Content=<translate><!--T:39--> 5- Launching FlyPi program: Putting it all together to start collecting image data!</translate><br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<translate></translate><br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Published<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=56884FlyPi2018-11-22T11:02:48Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=<translate><!--T:1--> FlyPi is a 'Do-It-Yourself' all in one biology lab tool that offers incredible versitility with optical microscopy, diagnostics, state of the art methods of research in neurosciences like optogenetics, calcium Imaging and fluorescence microscopy (Maia Chagas, 2017) among many other possibilities.</translate><br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=<translate><!--T:2--><br />
In this tutorial, I share the journey of how I built the FlyPi, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
<!--T:3--><br />
Steps<br />
<br />
<!--T:4--><br />
1- Understanding Modularity: How to decide which build is needed<br />
<br />
<!--T:5--><br />
2- Gathering materials & Creating parts: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
<!--T:6--><br />
3- Building PCB hardware: Quick overview of the PCB build. <br />
<br />
<!--T:8--><br />
4- Software Installation: How to install interface and useful imagej FIJI software on SD card<br />
<br />
<!--T:9--><br />
5- Launching the FlyPi: Putting it all together to start collecting image data!</translate><br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=<translate><!--T:10--><br />
Monitor <br />
<br />
<!--T:11--><br />
Computer mouse - USB<br />
<br />
<!--T:12--><br />
Keyboard - USB<br />
<br />
<!--T:13--><br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
<!--T:14--><br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )</translate><br />
|Tools=<translate><!--T:15--><br />
Soldering Iron, Solder<br />
<br />
<!--T:16--><br />
Computer with:<br />
<br />
<!--T:17--><br />
- SD card reader <br />
<br />
<!--T:18--><br />
- Internet Connection<br />
<br />
<!--T:19--><br />
3D printer</translate><br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:20--> Understanding Modularity</translate><br />
|Step_Content=<translate><!--T:21--><br />
1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
<!--T:22--><br />
The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. The modules in the PCB are Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
<!--T:23--><br />
To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).</translate><br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:24--> Gathering Materials & Creating Parts</translate><br />
|Step_Content=<translate><!--T:25--><br />
2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. <br />
* Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
* 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.</translate><br />
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:26--> Building PCB board</translate><br />
|Step_Content=<translate><!--T:27--><br />
The PCB board is organized in the following modules:<br />
<br />
<!--T:28--><br />
a) power 1 - 12v to 5v converter dedicated to power the Raspberry Pi<br />
<br />
<!--T:29--><br />
b) power 2 - 12v to 5v converter to power LEDs, LED ring, Thermistor and devices on the I2C port<br />
<br />
<!--T:30--><br />
c) High power LED / 5V general module 1 - Port that can be used with high power LEDs or any other component that takes up to 5V (uses a NPN transistor, PWM capable).<br />
<br />
<!--T:31--><br />
d) High power LED / 5V general module 2 - Port that can be used with high power LEDs or any other component that takes up to 5V.<br />
<br />
<!--T:32--><br />
e) LED ring module - used to power and control the LED ring<br />
<br />
<!--T:33--><br />
f) servo motor module - used to power and control a continuous servo motor<br />
<br />
<!--T:34--><br />
g) Peltier element module - comprised of fan, RGB led, thermistor and peltier element ports.<br />
<br />
<!--T:35--><br />
A fully populated port should look like the one in the image</translate><br />
|Step_Picture_00=FlyPi_all_modules.jpg<br />
|Step_Picture_00_annotation={"objects":[{"type":"textbox","originX":"center","originY":"center","left":353.99,"top":252,"width":95.97,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"A","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } },{"type":"textbox","originX":"center","originY":"center","left":335,"top":60,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"B","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } },{"type":"textbox","originX":"center","originY":"center","left":59,"top":44,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"C","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } },{"type":"textbox","originX":"center","originY":"center","left":202,"top":159,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"D","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } },{"type":"textbox","originX":"center","originY":"center","left":49,"top":183,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"E","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } },{"type":"textbox","originX":"center","originY":"center","left":58,"top":224,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"F","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} },"1":{"0":{} } } },{"type":"textbox","originX":"center","originY":"center","left":59,"top":334,"width":56,"height":22.6,"fill":"black","stroke":"black","strokeWidth":1,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":0,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"text":"G","fontSize":20,"fontWeight":"normal","fontFamily":"sans-serif","fontStyle":"","lineHeight":1.16,"textDecoration":"","textAlign":"left","textBackgroundColor":"","charSpacing":0,"minWidth":20,"styles":{"0":{"1":{} } } }],"height":"571","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_20181106_162732387.jpg<br />
|Step_Picture_02=FlyPi_IMG_20181106_165211238.jpg<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:36--> Adding Modules</translate><br />
|Step_Content=<translate><!--T:37--> 4- Software Installation: How to install useful software on SD card</translate><br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=<translate><!--T:38--> Launching the FlyPi</translate><br />
|Step_Content=<translate><!--T:39--> 5- Launching FlyPi program: Putting it all together to start collecting image data!</translate><br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<translate></translate><br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Published<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=55906FlyPi2018-11-07T07:57:07Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab tool that offers incredible versitility with optical microscopy, diagnostics, state of the art methods of research in neurosciences like optogenetics, calcium Imaging and fluorescence microscopy (Maia Chagas, 2017) among many other possibilities.<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Understanding Modularity: How to decide which build is needed<br />
<br />
2- Gathering materials & Creating parts: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building PCB hardware: Quick overview of the PCB build. Note the space left due to the absence of the servo motor for the (x,y) axis movement.<br />
<br />
4- Combining 3D printed body: Modular flexibility in use.<br />
<br />
5- Software Installation: How to install interface and useful imagej FIJI software on SD card<br />
<br />
6- Launching the FlyPi: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Understanding Modularity<br />
|Step_Content=1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. The modules in the PCB are Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).<br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. <br />
* Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
* 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.<br />
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building PCB board<br />
|Step_Content=3- Building PCB board:<br />
|Step_Picture_00=FlyPi_IMG_7773.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Adding Modules<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=55905FlyPi2018-11-07T07:34:04Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi biology tool, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Understanding Modularity: How to decide which build is needed<br />
<br />
2- Gathering materials & Creating parts: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building PCB hardware: Quick overview of the PCB build. Note the space left due to the absence of the servo motor for the (x,y) axis movement.<br />
<br />
4- Combining 3D printed body: Modular flexibility in use.<br />
<br />
5- Software Installation: How to install interface and useful imagej FIJI software on SD card<br />
<br />
6- Launching the FlyPi: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Understanding Modularity<br />
|Step_Content=1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
The FlyPi is designed in a modular fashion to optimize diverse capabilities. The base pieces are the raspberry pi, raspberry pi cam, the PCB with the Arduino and the 3D printed housing. The raspberry pi acts as the computer for the camera and the PCB/Arduino controls the mechanical functions of the modules. The modules in the PCB are Peltier module (temperature controlled plate), the multicolour LED ring, the UV light, the fans for the UV light and Peltier, the thermostat and the indication LED for the Peltier. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).<br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. <br />
* Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
* 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.<br />
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building PCB board<br />
|Step_Content=3- Building PCB board:<br />
|Step_Picture_00=FlyPi_IMG_7773.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Adding Modules<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=55903FlyPi2018-11-06T10:01:57Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi biology tool, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its wonderful new perspectives and illuminates minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility to research and explore the microscopic world around us. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Understanding Modularity: Loosely combined Parts can be comb<br />
<br />
2- Gathering materials & Creating parts: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building PCB hardware: Quick overview of the PCB build. Note the space left due to the absence of the servo motor for the (x,y) axis movement.<br />
<br />
4- Combining 3D printed body: Modular flexibility in use.<br />
<br />
5- Software Installation: How to install interface and useful imagej FIJI software on SD card<br />
<br />
6- Launching the FlyPi: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Understanding Modularity<br />
|Step_Content=1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Examples of modularity are things like cars, computers, solar panels, lego blocks and biological cells. Principles behind modularity posit that systems should be built from cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
The FlyPi is designed in a modular fashion to optimize diverse capabilities. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
To begin, I determined which modules I wanted to have. I decided to keep all but the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient. I also knew I wanted to customize the design for an (x,y,z) axis movement to a hydraulic syringe that could be manually manipulated(that's another tutorial).<br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials & Creating Parts: Not all parts are necessary to order for basic functionality. The repository has a detailed bill of materials that can be tailored to specific needs. <br />
* Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
* 3D print files can be found in the files tab above. I started printing at the local fablab (fabrication laboratory) after ordering the parts I needed to build the microscope. This way I would have the body ready when the electronics arrived.<br />
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building PCB board<br />
|Step_Content=3- Building PCB board:<br />
|Step_Picture_00=FlyPi_IMG_7773.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Adding Modules<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=55898FlyPi2018-11-05T18:26:11Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi biology tool, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new wonderful perspectives and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: Loosely combined Parts can be comb<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Modularity<br />
|Step_Content=1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Things like cars, computers, solar panels, biological cells are all examples of modularity. Principles behind modularity posit that systems should be built form cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
The FlyPi is designed in a modular fashion to optimize its diversity in capabilities. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
To begin, I determined which modules I wanted to have and left out the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient.<br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: Due to modularity, not all parts are necessary for basic functionality. (The repository has detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab above.<br />
|Step_Picture_00=FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building PCB board<br />
|Step_Content=3- Building PCB board:<br />
|Step_Picture_00=FlyPi_IMG_7775.JPG<br />
|Step_Picture_01=FlyPi_IMG_7757.JPG<br />
|Step_Picture_02=FlyPi_IMG_7770.JPG<br />
|Step_Picture_03=FlyPi_IMG_7773.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Adding Modules<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7773.JPG&diff=55897Fichier:FlyPi IMG 7773.JPG2018-11-05T18:23:17Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7770.JPG&diff=55896Fichier:FlyPi IMG 7770.JPG2018-11-05T18:21:58Z<p>TinkererTalley : TinkererTalley a téléversé une nouvelle version de Fichier:FlyPi IMG 7770.JPG</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7757.JPG&diff=55895Fichier:FlyPi IMG 7757.JPG2018-11-05T18:21:42Z<p>TinkererTalley : TinkererTalley a téléversé une nouvelle version de Fichier:FlyPi IMG 7757.JPG</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7775.JPG&diff=55894Fichier:FlyPi IMG 7775.JPG2018-11-05T18:20:03Z<p>TinkererTalley : TinkererTalley a téléversé une nouvelle version de Fichier:FlyPi IMG 7775.JPG</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7775.JPG&diff=55893Fichier:FlyPi IMG 7775.JPG2018-11-05T18:15:11Z<p>TinkererTalley : TinkererTalley a téléversé une nouvelle version de Fichier:FlyPi IMG 7775.JPG</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7747.JPG&diff=55892Fichier:FlyPi IMG 7747.JPG2018-11-05T18:01:52Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7757.JPG&diff=55891Fichier:FlyPi IMG 7757.JPG2018-11-05T18:01:42Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7760.JPG&diff=55890Fichier:FlyPi IMG 7760.JPG2018-11-05T18:01:28Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7770.JPG&diff=55889Fichier:FlyPi IMG 7770.JPG2018-11-05T18:01:15Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7771.JPG&diff=55888Fichier:FlyPi IMG 7771.JPG2018-11-05T18:01:01Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7775.JPG&diff=55887Fichier:FlyPi IMG 7775.JPG2018-11-05T18:00:48Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7776.JPG&diff=55886Fichier:FlyPi IMG 7776.JPG2018-11-05T18:00:35Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7777.JPG&diff=55885Fichier:FlyPi IMG 7777.JPG2018-11-05T18:00:22Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7788.JPG&diff=55884Fichier:FlyPi IMG 7788.JPG2018-11-05T18:00:11Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png&diff=55883Fichier:FlyPi Screen Shot 2018-11-05 at 17.34.04.png2018-11-05T17:58:38Z<p>TinkererTalley : TinkererTalley a téléversé une nouvelle version de Fichier:FlyPi Screen Shot 2018-11-05 at 17.34.04.png</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_Screen_Shot_2018-11-05_at_17.34.04.png&diff=55823Fichier:FlyPi Screen Shot 2018-11-05 at 17.34.04.png2018-11-05T16:34:48Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=55822FlyPi2018-11-05T16:30:06Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi biology tool, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new wonderful perspectives and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: Loosely combined Parts can be comb<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Modularity<br />
|Step_Content=1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use. Things like cars, computers, solar panels, biological cells are all examples of modularity. Principles behind modularity posit that systems should be built form cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
The FlyPi is designed in a modular fashion to optimize its diversity in capabilities. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
To begin, I determined which modules I wanted to have and left out the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient.<br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: Due to modularity, not all parts are necessary for basic functionality. (The repository has detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab<br />
<br />
The PCB board is custom made for Promethius Science<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building hardware<br />
|Step_Content=3- Building hardware: Explain module flexibilities<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Installation<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=55820FlyPi2018-11-05T09:14:44Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi biology tool, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new wonderful perspectives and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: Loosely combined Parts can be comb<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Modularity<br />
|Step_Content=1- Modularity: Broadly speaking, is the degree to which a system's components may be separated and recombined, often with the benefit of flexibility and variety in use.<sup id="cite_ref-MWModular_1-0" class="reference">[https://en.wikipedia.org/wiki/Modularity#cite_note-MWModular-1 <nowiki>[1]</nowiki>]</sup> Things like cars, computers, solar panels, biological cells are all examples of modularity. Principles behind modularity posit that systems should be built form cohesive loosely coupled components, sometimes referred to as modules, with a defined function or purpose. <br />
<br />
The FlyPi is designed in a modular fashion to optimize its diversity in capabilities. It's important to point out that all modules do not have to be added which can save time and money. If desired, modules can be added at any point. <br />
<br />
To begin, I determined which modules I wanted to have and left out the servo motor that provided some (x,y) axis movement for the slide. My samples were large enough that manual movement would be sufficient.<br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: Due to modularity, not all parts are necessary for basic functionality. (The repository has detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab<br />
<br />
The PCB board is custom made for Promethius Science<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building hardware<br />
|Step_Content=3- Building hardware: Explain module flexibilities<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Installation<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=52983FlyPi2018-09-04T15:36:57Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi biology tool, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new wonderful perspectives and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: Loosely combined Parts can be comb<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Modularity<br />
|Step_Content=1- Modularity: show some images/examples of the setup built, Explain Modularity principle<br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building hardware<br />
|Step_Content=3- Building hardware: Explain module flexibilities<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Installation<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=52982FlyPi2018-09-04T15:31:09Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=100<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi biology tool, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new wonderful perspectives and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: show some images/examples of the setup built, Explain Modularity principle .<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Step_Picture_00=FlyPi_IMG_7643.JPG<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Modularity<br />
|Step_Content=1- Modularity: show some images/examples of the setup built, Explain Modularity principle<br />
|Step_Picture_00=FlyPi_IMG_7835_1_.JPG<br />
|Step_Picture_00_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":409,"top":129,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":387,"top":149.96,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":2.13,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1.82,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":274.46,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.5,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":3,"scaleY":1,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":120,"top":356.44,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.55,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.88,"scaleY":1,"angle":-90,"flipX":true,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]},{"type":"wfarrow","originX":"center","originY":"center","left":492,"top":292,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":3,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":1,"scaleY":1,"angle":86.89,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_01=FlyPi_IMG_7891.JPG<br />
|Step_Picture_02=FlyPi_IMG_7774.JPG<br />
|Step_Picture_02_annotation={"objects":[{"type":"wfarrow","originX":"center","originY":"center","left":150.63,"top":370.99,"width":10,"height":90,"fill":"rgba(255,0,0,0)","stroke":"red","strokeWidth":1.31,"strokeDashArray":null,"strokeLineCap":"butt","strokeLineJoin":"miter","strokeMiterLimit":10,"scaleX":2.3,"scaleY":2.3,"angle":-90,"flipX":false,"flipY":false,"opacity":1,"shadow":null,"visible":true,"clipTo":null,"backgroundColor":"","fillRule":"nonzero","globalCompositeOperation":"source-over","transformMatrix":null,"skewX":0,"skewY":0,"points":[{"x":10,"y":10},{"x":10,"y":100},{"x":5,"y":90},{"x":10,"y":100},{"x":15,"y":90}]}],"height":"450","width":"600"}<br />
|Step_Picture_03=FlyPi_IMG_7802.JPG<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building hardware<br />
|Step_Content=3- Building hardware: Explain module flexibilities<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Installation<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7802.JPG&diff=52981Fichier:FlyPi IMG 7802.JPG2018-09-04T15:30:57Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7643.JPG&diff=52980Fichier:FlyPi IMG 7643.JPG2018-09-04T15:30:46Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7774.JPG&diff=52979Fichier:FlyPi IMG 7774.JPG2018-09-04T15:24:27Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7891.JPG&diff=52978Fichier:FlyPi IMG 7891.JPG2018-09-04T15:19:47Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7835_1_.JPG&diff=52977Fichier:FlyPi IMG 7835 1 .JPG2018-09-04T15:14:48Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=52370FlyPi2018-08-29T08:44:22Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=200<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=In this tutorial, I share the journey of how I built the FlyPi biology tool, without any previous electronics experience, so I could feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new wonderful perspectives and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators to look deeper into the micro world, is access to effective scientific tools. This is likely due to high prices set by development under a patent/scarcity methodology. Building your own FlyPi is a relatively easy and cost effective way to to get around this limiting factor. FlyPi is an all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, effective and low cost way to have better experimental control by customizing for specific needs and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: show some images/examples of the setup built, Explain Modularity principle .<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Modularity<br />
|Step_Content=1- Modularity: show some images/examples of the setup built, Explain Modularity principle<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building hardware<br />
|Step_Content=3- Building hardware: Explain module flexibilities<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Installation<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=52368FlyPi2018-08-29T08:19:43Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7640.JPG<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=200<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=The aim of this tutorial is to consolidate all construction information for this biology lab so I can feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new perspectives, joy, wonder and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators is access to effective scientific tools, likely due to high prices set by development under a patent/scarcity methodology. One way to get around this limiting factor is by building your own biology tools like the FlyPi; An all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, low cost device which allows for better experimental control, customization for specific needs, and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: show some images/examples of the setup built, Explain Modularity principle .<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Modularity<br />
|Step_Content=1- Modularity: show some images/examples of the setup built, Explain Modularity principle<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building hardware<br />
|Step_Content=3- Building hardware: Explain module flexibilities<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Installation<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7640.JPG&diff=52367Fichier:FlyPi IMG 7640.JPG2018-08-29T08:19:10Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=52366FlyPi2018-08-29T08:02:27Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7827.jpg<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=200<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=The aim of this tutorial is to consolidate all construction information for this biology lab so I can feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new perspectives, joy, wonder and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators is access to effective scientific tools, likely due to high prices set by development under a patent/scarcity methodology. One way to get around this limiting factor is by building your own biology tools like the FlyPi; An all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, low cost device which allows for better experimental control, customization for specific needs, and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: show some images/examples of the setup built, Explain Modularity principle .<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Modularity<br />
|Step_Content=1- Modularity: show some images/examples of the setup built, Explain Modularity principle<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Building hardware<br />
|Step_Content=3- Building hardware: Explain module flexibilities<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Installation<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Launching the FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=52365FlyPi2018-08-29T08:00:43Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7827.jpg<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods of research in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=200<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=The aim of this tutorial is to consolidate all construction information for this biology lab so I can feed my insatiable urge to see how things work. Microscopy offers a lens to explore each visual frontier with its new perspectives, joy, wonder and illumination of a minuscule life regularly unobserved. This is an amazing experiences for anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and/or educators is access to effective scientific tools, likely due to high prices set by development under a patent/scarcity methodology. One way to get around this limiting factor is by building your own biology tools like the FlyPi; An all-in-one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, low cost device which allows for better experimental control, customization for specific needs, and most importantly enables accessibility for exploration and research of the micro world around. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join scientific efforts in pursuing solutions to their local problems! <br />
<br />
Steps<br />
<br />
1- Modularity: show some images/examples of the setup built, Explain Modularity principle .<br />
<br />
2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- Building hardware: Explain module flexibilities<br />
<br />
4- Software Installation: How to install useful software on SD card<br />
<br />
5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Material=Monitor <br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl) : Under 'Files' tab above<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron, Solder<br />
<br />
Computer with:<br />
<br />
- ability to connect SD card <br />
<br />
- internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Step 1 : Modularity<br />
|Step_Content=1- Modularity: show some images/examples of the setup built, Explain Modularity principle<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Step 2 : Gathering Materials & Creating Parts<br />
|Step_Content=2- Gathering materials: - Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts):<br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/<br />
<br />
3D print files can be found in the files tab<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Step 3 : Building hardware<br />
|Step_Content=3- Building hardware: Explain module flexibilities<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Step 4 : Software Installation<br />
|Step_Content=4- Software Installation: How to install useful software on SD card<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Step 5 : Launching FlyPi<br />
|Step_Content=5- Launching FlyPi program: Putting it all together to start collecting image data!<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=49548FlyPi2018-07-28T09:47:00Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7827.jpg<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi is a 'Do-It-Yourself' all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=200<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=I started this project because I have an insatiable appetite to see how things work. Microscopy offers a lens to explore each visual frontier with its new perspectives, joy, wonder and understanding of a minuscule life usually unobserved. It is one of the most amazing experiences to be able to bring that to anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and educators is access to appropriate scientific tools. Likely due to high prices, set by development under a patent/scarcity methodology. One way to solve this is to Do-It-Yourself with the FlyPi, an all in one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). It's modularity offers a fast, low cost device which allows better experimental control, customization for specific needs, and most importantly enables accessibility to get more people into exploring the micro world around them. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join the scientific efforts and to pursue solutions to their local problems! <br />
<br />
Step 1 - order the parts needed for the PCB (kitspace) (should I explain the modules here? what if they don't need a peltier module for example?)<br />
<br />
2- collect 3D files and start printing the needed parts<br />
<br />
3- gathering hardware to put together (sub section on explaining the modules)<br />
<br />
4- installing software<br />
<br />
5- putting it all together to collect imaging data<br />
<br />
I shared the tutorial with Prometheus Science and it seems to come up under projects in the group. [[FlyPi|http://wikifab.org/wiki/FlyPi#]]<br />
<br />
Andre’s Suggestions:<br />
<br />
1- show some images/examples of the setup built and explain the modularity principle.<br />
<br />
2- Show the options to buy parts from kitspace, but also add links to the original github repository, stating that due to modularity, not all parts are necessary for basic functionality. (on the repository there are more detailed bill of material files)<br />
<br />
3- gathering hardware to put together (sub section on explaining the modules)<br />
<br />
4- installing software<br />
<br />
5- putting it all together to collect imaging data<br />
}}<br />
{{ {{tntn|Materials}}<br />
|Material=Monitor<br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl):<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron<br />
<br />
Internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Hex.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBRingHolder.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Collect Materials Needed<br />
|Step_Content=Electronic parts can be ordered from this kitspace page (lists have been conveniently compiled in various online market shopping carts): <br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/ <br />
<br />
3D print files can be found in the files tab<br />
|Step_Picture_00=FlyPi_IMG_7615.jpg<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Upload and Assembly<br />
|Step_Content=<br />
}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Software Upload and Assembly<br />
|Step_Content=<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:FlyPi_IMG_7615.jpg&diff=47570Fichier:FlyPi IMG 7615.jpg2018-07-19T14:28:43Z<p>TinkererTalley : File uploaded with MsUpload on FlyPi</p>
<hr />
<div>File uploaded with MsUpload on [[FlyPi]]</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:RGBRingHolder.stl&diff=47569Fichier:RGBRingHolder.stl2018-07-19T14:13:20Z<p>TinkererTalley : TinkererTalley a téléversé une nouvelle version de Fichier:RGBRingHolder.stl</p>
<hr />
<div></div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:RGBRingHolder.stl&diff=47568Fichier:RGBRingHolder.stl2018-07-19T14:08:56Z<p>TinkererTalley : </p>
<hr />
<div></div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:RGBHeatSink_Hex.stl&diff=47567Fichier:RGBHeatSink Hex.stl2018-07-19T14:08:06Z<p>TinkererTalley : </p>
<hr />
<div></div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=47162FlyPi2018-07-13T09:30:10Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7827.jpg<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi - DIY all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=200<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=I started this project because I have an insatiable appetite to see how things work. Microscopy offers a lens to explore each visual frontier with its new perspectives, joy, wonder and understanding of a minuscule life usually unobserved. It is one of the most amazing experiences to be able to bring that to anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and educators is access to appropriate scientific tools. Likely due to high prices, set by development under a patent/scarcity methodology. One way to solve this is to DIY with the FlyPi, an all in one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). Other than the low cost, this device allows better experimental control, customization for specific needs, and most importantly enables accessibility to get more people into exploring the micro world around them. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join the scientific efforts and to pursue solutions to their local problems! <br />
}}<br />
{{ {{tntn|Materials}}<br />
|Material=Monitor<br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl):<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron<br />
<br />
Internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Collect Materials Needed<br />
|Step_Content=Electronic parts can be ordered from this kitspace page where the lists have been compiled in various online market shopping carts for convenience. <br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/ <br />
<br />
3D print files:<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=47161FlyPi2018-07-13T09:18:36Z<p>TinkererTalley : Saved using "Save and continue" button in form</p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7827.jpg<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi - DIY all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=200<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=I started this project because I have an insatiable appetite to see how things work. Microscopy offers a lens to explore each visual frontier with its new perspectives, joy, wonder and understanding of a minuscule life usually unobserved. It is one of the most amazing experiences to be able to bring that to anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and educators is access to appropriate scientific tools. Likely due to high prices, set by development under a patent/scarcity methodology. One way to solve this is to DIY with the FlyPi, an all in one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). Other than the low cost, this device allows better experimental control, customization for specific needs, and most importantly enables accessibility to get more people into exploring the micro world around them. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join the scientific efforts and to pursue solutions to their local problems! <br />
}}<br />
{{ {{tntn|Materials}}<br />
|Material=Monitor<br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl):<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron<br />
<br />
Internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle Thread.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=NeckTriangle.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Arm.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RGBHeatSink Frame.stl<br />
}}{{ {{tntn|Tuto Attachments}}}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Collect Materials Needed<br />
|Step_Content=Electronic parts can be ordered from this kitspace page where the lists have been compiled in various online market shopping carts for convenience. <br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/ <br />
<br />
3D print files:<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:RGBHeatSink_Frame.stl&diff=47160Fichier:RGBHeatSink Frame.stl2018-07-13T09:15:42Z<p>TinkererTalley : </p>
<hr />
<div></div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:RGBHeatSink_Arm.stl&diff=47159Fichier:RGBHeatSink Arm.stl2018-07-13T09:14:04Z<p>TinkererTalley : </p>
<hr />
<div></div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:NeckTriangle.stl&diff=47158Fichier:NeckTriangle.stl2018-07-13T09:10:08Z<p>TinkererTalley : </p>
<hr />
<div></div>TinkererTalleyhttps://wikifab.org/w/index.php?title=Fichier:NeckTriangle_Thread.stl&diff=47157Fichier:NeckTriangle Thread.stl2018-07-13T09:08:50Z<p>TinkererTalley : </p>
<hr />
<div></div>TinkererTalleyhttps://wikifab.org/w/index.php?title=FlyPi&diff=47156FlyPi2018-07-13T08:58:23Z<p>TinkererTalley : </p>
<hr />
<div>{{ {{tntn|Tuto Details}}<br />
|Main_Picture=FlyPi_IMG_7827.jpg<br />
|Licences=Attribution (CC BY)<br />
|Description=FlyPi - DIY all in one biology lab that can be used for optical microscopy, diagnostics, perform state of the art methods in Neurosciences, such as Optogenetics, Calcium Imaging and Fluorescence microscopy (more details can be found in Maia Chagas, 2017).<br />
|Area=Science and Biology<br />
|Type=Creation<br />
|Difficulty=Medium<br />
|Duration=28<br />
|Duration-type=hour(s)<br />
|Cost=200<br />
|Currency=EUR (€)<br />
|Tags=DIY microscope, flourescence, arduino, open source hardware, thermogenetics, raspberry Pi, optogenetics, opensource software, behavior, research, citizen science, microscopy, behavioral tracking, behavioral research, flourescent imaging, calcium imaging, LED filters, 3D printing, github repository, PCB soldering, photography, exploring<br />
|SourceLanguage=none<br />
|Language=en-gb<br />
|IsTranslation=0<br />
}}<br />
{{ {{tntn|Introduction}}<br />
|Introduction=I started this project because I have an insatiable appetite to see how things work. Microscopy offers a lens to explore each visual frontier with its new perspectives, joy, wonder and understanding of a minuscule life usually unobserved. It is one of the most amazing experiences to be able to bring that to anyone curious of what lies beneath our immediate visual capabilities. One main constraint for curious students, researchers and educators is access to appropriate scientific tools. Likely due to high prices, set by development under a patent/scarcity methodology. One way to solve this is to DIY with the FlyPi, an all in one biology lab with powerful “off-the-shelf” electronic elements (Raspberry Pi & Arduino nano). Other than the low cost, this device allows better experimental control, customization for specific needs, and most importantly enables accessibility to get more people into exploring the micro world around them. Just putting it together is a great learning experience for everyone not immediately comfortable with electronics. Some People have even begun to join the scientific efforts and to pursue solutions to their local problems! <br />
}}<br />
{{ {{tntn|Materials}}<br />
|Material=Monitor<br />
<br />
Computer mouse - USB<br />
<br />
Keyboard - USB<br />
<br />
3D print files (.stl):<br />
<br />
Custom PCB (Principle Component Board parts can be found on: https://kitspace.org/boards/github.com/prometheus-science/flypi/ )<br />
|Tools=Soldering Iron<br />
<br />
Internet Connection<br />
<br />
3D printer<br />
|Tuto_Attachments={{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base Clip.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Base.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Cap.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Camera Housing.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=CameraServoHolder.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightDiffuser.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm Cross2.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=LightFilter Arm.STL<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=ManipulatorBase.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck L.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=Neck R.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=PCBCase Top.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Bot.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Rails.stl<br />
}}{{ {{tntn|Tuto Attachments}}<br />
|Attachment=RaspberryCase Top.stl<br />
}}<br />
}}<br />
{{ {{tntn|Separator}}}}<br />
{{ {{tntn|Tuto Step}}<br />
|Step_Title=Collect Materials Needed<br />
|Step_Content=Electronic parts can be ordered from this kitspace page where the lists have been compiled in various online market shopping carts for convenience. <br />
<br />
https://kitspace.org/boards/github.com/prometheus-science/flypi/ <br />
<br />
3D print files:<br />
}}<br />
{{ {{tntn|Notes}}<br />
|Notes=<br />
}}<br />
{{ {{tntn|Tuto Status}}<br />
|Complete=Draft<br />
}}</div>TinkererTalley